![]() Simple Western analysis of lysates (0.1 mg/mL) from HeLa cells using -Actin (13E5) Rabbit mAb 4970. Western blot analysis of recombinant Actin isoforms using -Actin (13E5) Rabbit mAb (upper) and Pan-Actin Antibody 4968 (lower). Target Information Beta actin is one of six actin isoforms which have been identified. Western blot analysis of cell extracts from various cell lines using beta-Actin (13E5) Rabbit mAb. Anti-beta Actin antibody (ab8227) ab8227 staining beta Actin in NIH/3T3. For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required. Tested in Western Blot (WB) and Immunocytochemistry (ICC/IF) applications. For western blotting, we have tested and recommend using the antibody at a concentration of 500 ng/ml (1:1000 dilution). Western Blot Analysis: 1:500 dilution of this lot detected ACTIN on 10 ug of A431 lysates. This highly published monoclonal antibody is validated for use in ELISA, IC, IF, IH, IH(P) & WB. Signal intensity was normalized to the tubulin signal. By Western blot, PA1-183 detects a prominent 42kD protein in human, mouse, non-human primate, and rat whole cell lysates. Question 2: What dilution do you recommend for the polyclonal anti-actin antibody Answer 2: The dilution of antibody depends on the cell/tissue type and experimental format being used. Reliably and specifically detect actin using this Anti-Actin Antibody, clone C4. Normalized signal intensity of Actin Beta from whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against Actin Beta (1 & 2) or a scrambled siRNA (-). Western blot analysis of whole cell lysates prepared from HeLa separately transfected with two unique siRNA duplexes against Actin Beta (1 & 2) or a scrambled siRNA (-) probed with Mouse anti Actin Beta antibody ( VMA00048) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-tubulin primary antibody ( 12004165) and visualized on the ChemiDoc MP with an 50 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure. Arrow points to actin beta (molecular weight 42 kDa). Western blot - Anti-beta Actin antibody (ab8227) All lanes : Anti-beta Actin antibody (ab8227) at 0. Actin Antibody Sigma, Anti Actin, Anti Actin Antibody Western Blot, Anti Actin Antibody for western blot - Anti-Actin antibody produced in rabbit, Anti Actin Sigma, Sigma Actin Antibody MDL number: MFCD00145889 NACRES: NA. ![]() Western blot analysis of whole cell lysates probed with actin beta antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 25 second exposure. GenScript MonoRab Beta-Actin Antibody, mAb, Rabbit reacts with mouse, human, hamster, and rat. ![]()
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